U2-S03. Preparation of Bioconjugates and Molecular Probes

Preparation of Bioconjugates and Molecular Probes

The preparation of bioconjugates and molecular probes involves a diverse array of techniques and strategies aimed at functionalizing biomolecules for various applications. This process encompasses the labeling of antibodies, haptenized proteins, and enzymes, as well as the biotinylation and fluorescent labeling of probes, and the conjugation of biomolecules with nanoparticles and other entities.

Customer benefits

• To develop antibodies against small molecules (preparation of immunization bioconjugate)
• Enhanced Functionality: Bioconjugation allows for specific binding of biomolecules, such as antibodies or peptides, to other entities like molecular probes or nanoparticles. This expands the applications and functionalities of biomolecules.
• Improved Sensitivity and Specificity: Bioconjugates can enhance the sensitivity and specificity of detection techniques, such as immunohistochemistry or flow cytometry.
• Diverse Applications: Bioconjugates find application in research, diagnostics, and therapy, benefiting research laboratories, hospitals, and pharmaceutical companies.

Target customer

• Researchers and Scientists: Satisfy the need to label biomolecules for research studies.
• Pharmaceutical and Biotechnology Companies: For creating therapeutic bioconjugates, imaging markers or bioconjugates for immunizations of non-immunogenic small molecules, between other.

Additional information

Selected references:

• E. Montagut, J. Raya, M.-T. Martín Gómez, L. Vilaplana, B. Rodríguez-Urretavizcaya, M.-P. Marco. An Immunochemical Approach to detect the Quorum Sensing-Regulated Virulence Factor 2-Heptyl-4-Quinoline N-Oxide (HQNO) produced by Pseudomonas aeruginosa Clinical Isolates. Microbiol. Spect., 10(4), 1-12, 2022.
• B. Rodriguez-Urretavizcaya, N. Pascual, C. Pastells, M. T. Martin-Gomez, Ll. Vilaplana, M.-P. Marco. Diagnostic and Stratification of Pseudomonas aeruginosa Infected Patients by Immunochemical Quantitative Determination of Pyocyanin from Clinical Bacterial Isolates. Frontiers in Cell. Infect. Microbiol., 11, 786929, 2021. DOI: 10.3389/fcimb.2021.786929.
• J. Marrugo-Ramírez, M. Rodríguez-Núñez, M.-P Marco, M. Mir, J. Samitier. Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease. Biosensors, 11(1), 20, 2021.
• E. J. Montagut, Ll. Vilaplana, M.T. Martin-Gómez, M.-P. Marco. A High Throughput Immunochemical Method to Assess 2-Heptyl-4-Quinolone Quorum Sensing Molecule as Potential Biomarker. ACS Infect. Dis., 6(12), 3237-3246, 2020.
• M. Broto, R. McCabe, R. Galve, M.-P. Marco. A high-specificity immunoassay for the therapeutic drug monitoring of ciclophosphamide. Analyst, 144, 5172-5178, 2019.