U2-S06. In vitro antibody production

In vitro antibody production

We perform the production of monoclonal antibodies in vitro.
Hybridoma cells are first expanded in culture flask or high density culture devices (for larger amounts of antibody) to get the optimal cell density needed for the production.
The classical medium of production includes fetal calf serum (ultralow IgG, FCS) but our expertise allows us to provide a completely customized service according to your needs, without using FCS.
Supernatant containing the monoclonal antibodies is then periodically collected until the expected quantity of antibodies is obtained.

Depending on the nature of the sample, the expected antibody concentrations are for :­

• Culture in flask: from 5 to 20 µg antibody/mL supernatant
• ­ Culture in high density device : up to 1 mg antibody/mL supernatant

Customer benefits

• Customization: The service can be tailored to meet the specific needs of each customer, allowing for personalized antibody production without using fetal calf serum (FCS).
• Scalability: The service can accommodate both flask cultures and high-density devices, providing flexibility for different antibody quantities.
• ISO 9001 Certification: We operate under ISO 9001:2015 quality standards, ensuring consistent and reliable antibody production.

Target customer

• Research Institutions: Universities, research centers, and laboratories engaged in biomedical research.
• Biotech Companies: Organizations involved in drug discovery, diagnostics, and therapeutic development.
• Pharmaceutical Companies: Those working on antibody-based therapies or vaccines.
• Healthcare Providers: Hospitals and clinics conducting specialized research.
• Food & Environmental Quality Control Companies: Entities involved in:
  • Food Contaminant Detection: Developing antibody-based tests for identifying foodborne pathogens and toxins.
  • Environmental Contaminant Detection: Creating antibody-based assays for detecting environmental pollutants in air, water, and soil.

Additional information

Selected references:

• Giovanna Roncador; Pablo Engel; Lorena Maestre; et al; Alison H.Banham., Nuria Pascual 2016. The European antibody network’s practical guide to finding and validating suitable antibodies for research. mAbs. Taylor & Francis Online. 8-1, pp.27-36
• Rodriguez-Urretavizcaya, N. Pascual, C. Pastells, M. T. Martin-Gomez, Ll. Vilaplana, M.-P. Marco. Diagnostic and Stratification of Pseudomonas aeruginosa Infected Patients by Immunochemical Quantitative Determination of Pyocyanin from Clinical Bacterial Isolates. Frontiers in Cell. Infect. Microbiol., 11, 786929, 2021. DOI: 10.3389/fcimb.2021.786929